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Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination

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Scientific Article | Ce protocole décrit en détail la préparation des complexes nucléosomiques à l’aide de deux méthodes de préparation des échantillons
DNA repair in the context of chromatin is poorly understood. Biochemical studies using nucleosome core particles, the fundamental repeating unit of chromatin, show most DNA repair enzymes remove DNA damage at reduced rates as compared to free DNA.

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Molecular organization of the early stages of nucleosome phase

Preparation of Nucleosome Core Particles Complexed with DNA Repair

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Cryo-EM structure of SWI/SNF complex bound to a nucleosome

Structural basis for binding diversity of acetyltransferase p300

Preparation of Nucleosome Core Particles Complexed with DNA Repair

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Correlating histone acetylation with nucleosome core particle

Cryo-EM structures of RNA polymerase II-nucleosome complexes

Nucleotide excision repair leaves a mark on chromatin: DNA damage

The cryo‐EM structure of the CENP‐A nucleosome in complex with

Preparation of Nucleosome Core Particles Complexed with DNA Repair

Frontiers Histone Modifications, Internucleosome Dynamics, and